Fibro-Plus (PRP Augmented Autologous Fibroblast Grafting)


(PRP Augmented Autologous Fibroblast Grafting)


PRP Augmented Autologous Fibroblast Grafting

Skin Tissue and Fibroblasts

Fibroblasts are connective tissue cells that synthesize collagen and other intercellular matrix components. Therefore, they play a role in the structuring, nourishment, and regeneration of the skin.

What is Fibro-Plus?

Fibro-Plus is a Cellular Therapy method utilizing live fibroblasts, which are cultured using the patient’s own cells obtained through PRP and serum derived from the patient’s own blood, effectively reversing the effects of time.


Autologous use refers to the reutilization of organs, tissues, and cells obtained from the individual’s body. Autologous use eliminates the risk of tissue rejection and prevents the transmission of undesired microbiological agents (bacteria, viruses, prions, etc.) to the recipient.

How is Fibro-Plus different from other Fibroblast cultures?

Autologous Fibroblasts can be augmented using PRP (Platelet Rich Plasma) to enhance their effectiveness and quantity. Platelets, rich in growth factors, accelerate tissue healing.


The preparation and multiplication of Autologous Fibroblasts are done using PRP and serum derived from the patient’s own blood. Consequently, this approach provides a more successful, effective, and risk-free application compared to using Fibroblasts alone.

Why Autologous Fibroblasts?

The main advantage of Autologous Fibroblast application lies in the triggering of collagen production and rejuvenation, which happens more rapidly and effectively by adding PRP during the preparation stage of Fibroblasts.


The individual’s cells not only produce new collagen but also contribute to the renewal and repair of the skin.

Autologous Fibroblast Grafting

The process involves purifying and culturing fibroblasts obtained from the patient’s skin tissue via biopsy in a laboratory environment. These cultured fibroblasts are then injected back into problematic areas of the skin.


The expected indication is that fibroblasts dispersed in physiological solution, transferred to problematic areas using an ‘insulin injector,’ will proliferate, producing regenerative agents for the skin and reversing observed damage prior to the application.


Biopsy – Cell Culture – Quality Tests – Injection


Where Can It Be Applied?

  • For Lip Augmentation
  • To Eliminate Facial Wrinkles
  • For the Treatment of Hollows Due to Burns, Previous Surgeries, or Trauma
  • Facial Rejuvenation Procedures that can be done with Mesotherapy
  • To Correct Deformities Resulting from Conditions like Acne or Chickenpox
  • For Treating Persistent Chronic Wounds
  • For Treating Long-standing Skin Scars

What Are the Advantages Compared to Other Methods?

Autologous Fibroblast Injection is not merely a short-term solution like the application of filler materials, but rather a method used for treating skin wrinkles that has long-term effects.

  1. Fibro-Plus Is Absolutely Non-Allergic.

Due to the use of Autologous serum and PRP to prepare PRP-supported Autologous Fibroblasts, there are no reactions or attempts by the body to eliminate or expel any foreign substances.


  1. No Risk of Animal Diseases

In this procedure, besides the use of autologous tissue, the serum obtained from this tissue is derived from the patient’s own blood. Animal serums sold commercially for cell cultures are not utilized in our laboratory.


  1. It’s Long-lasting and Highly Effective

Numerically multiplied live autologous fibroblasts, when injected into the targeted area for treatment, establish a continuous protein repair system. The latest findings in literature demonstrate measurable improvements lasting between 12 to 48 months. Histological analyses in these studies confirm that fibroblast injection increases collagen and dermal collagen density.

Who Should Consider Autologous Fibroblast Injections?

Application between ages 30-45 provides the most effective results. Applications can also be performed for patients over 45; however, the effect duration is slightly longer compared to the 30-45 age group. Besides the natural deformation of the skin, advanced acne, scars, and deformities caused by infection can be treated regardless of age.

Are There Any Side Effects?

This procedure utilizes Autologous (the patient’s own cells) cells in development, hence, as Autologous serum is also used, there are no side effects.


A 2x4mm tissue sample is obtained from behind the ear (preferably the earlobe) or the inner surface of the arm, where dense hair follicles are absent, via a punch biopsy or an excisional biopsy. The area is previously anesthetized both locally and topically.


Since the procedure involves cutting through the upper layer of fatty dermis, stitches might be necessary to close the wound. Subsequently, a dressing or an adhesive bandage is applied to the biopsy area to protect the wound and prevent bleeding.


Due to the high likelihood of scarring in punch biopsies, excisional biopsies are more commonly preferred.


Prior to the Biopsy day, Genkord ensures the transfer of the sample using a kit. Our kit consists of 1 pediatric blood bag, a tube containing preservative liquid, an information form, and a sterile biopsy punch for use during the biopsy.


Approximately 100cc of the patient’s blood is drawn. The collected biopsy samples should be placed into the preservative liquid provided by us.

How is the Fibro-Plus Application Carried Out? Does It Cause Pain?

When the desired cell density of approximately 7-10×10^6 per ml is reached after around 1 month, the fibroblast cells are filled into a syringe (minimum 2cc) in a sterile environment and delivered to the physician through cold-chain transportation.


The application involves an injection into the skin. Prior to the application, anesthesia is applied using a local anesthetic cream. However, depending on the pain threshold of patients, slight discomfort might be felt.

Post-Application Care

Normal daily life can be continued after the application. Unlike alternative treatments, there are no complaints of swelling or pain in the facial area. It is advised not to wear heavy makeup on the day of the application and avoid exposing the treated area to sunlight for 15 days.

How Do We Ensure Quality Control?

  • Cell count: The target is to have 7-10x〖10〗^6 cells per ml.
  • Viability (presence of over 95% live cells)
  • Morphological analysis: Particularly the presence of spindle cells is preferred, as they signify the beginning of cell differentiation. Polygonal cells are considered as cells that have started to differentiate.
  • Immunophenotyping: Surface markers specific to fibroblasts are tested for their presence.
  • Differentiation capacity measurement: Differentiation studies into adipocytes, cartilage, smooth muscle, neurons, and endothelial cells are recommended to determine the differentiation capability across all three germ layers of the produced cells.
  • Aerobic and anaerobic cultures: A 14-day culture process monitoring is mandatory in human applications.

What Should Be Considered When Acquiring the Service?

As Autologous Fibroblast and PRP applications involve the use of tissues and blood obtained from the individual, the products must be prepared in laboratories with GMP standards and GMP equipment and applied by a specialist physician.


Freezing and Storage

Within the scope of GENKORD cell banking, we provide a service to store patients’ cells for future use, if desired.


The numerically increased cells are preserved in -196°C liquid nitrogen tanks by adding special solutions, ensuring their preservation for several years.


On the day of application, they are thawed and made ready for the procedure.